LocIn | A targeted integration system for the efficient creation of isogenic mammalian cell lines

Watch our latest Labroots webinar on LocIn (pronounced “Loch-In”) where our Director of Molecular Biology and Cell Engineering, Dr Tim London presents this new innovative targeted integration system. 

Key points include:

• Learn about cell lines and their importance in drug development
• Learn about traditional cell line generation timelines and how they compare to our fast-tracked LocIn system
• Discover how LocIn, this novel targeted integration system works
• Learn what Recombinase Mediated Cassette Exchange is and how it can be used to retarget pre-defined genomic locations (landing pads) for the rapid generation of isogenic cell lines. 
• Learn how constitutive EGFP expression from the platform LocIn cell lines means it is possible to assess expression (high/medium/low) potential before retargeting is performed. 
• Discover how LocIn’s unique counter selection system ensures that all landing pads are retargeted. Learn how LocIn allows the generation of clonal cell populations without the need for cloning out.

What is LocIn?

LocIn is an advanced targeted integration system created by RoukenBio designed for the efficient generation of cell lines by retargeting pre-defined genomic loci, known as "landing pads." These landing pads incorporate a fluorescent protein marker, enabling the easy identification of integrations at genomic sites that support high transgene expression. 

The system leverages Recombinase Mediated Cassette Exchange (RMCE) to precisely replace the landing pad cassette with a gene of interest. This approach ensures the complete removal of the original cassette during retargeting while exclusively integrating the desired gene. By eliminating positional and context-dependent effects, LocIn enables accurate studies of phenotypic outcomes, delivering reliable genotype-to-phenotype correlations.

How can LocIn take cell engineering to the next level?

LocIn was developed with clear differentiators in mind which help drug developers and biotherapeutic innovators benefit from:

• Speed – By using LocIn, you achieve faster cell line engineering as we cut out the time-consuming sub-cloning step from the traditional cell line generation timeline.
• Uniformity – We get consistent results every time, with the same integration pattern across all cell lines ensuring uniformity.
• Control – Reduce integration site artefacts and variability for more reliable results.
• Flexible platforms – Choose from single or multi-platform systems for low or high expression needs, with complete control over your cell line generation process.

Read more on how the LocIn targeted integration system works and see how its single and multi-platform systems differ.

Implications of LocIn for drug discovery 

LocIn is a powerful tool transforming the traditional timelines of cell line generation making it less time consuming and more efficient. This has applications for testing target molecules across various therapeutic areas, like oncology, immuno-oncology, autoimmune diseases, allergy, and inflammatory conditions.