Cell Lines

We specialise in creating cell lines for all stages of therapeutic development; cell-based bioassays for screening, selection, functional assessments, potency assays and lot release. Our expert team employ a range of molecular tools to create custom genetically engineered cell lines to specification and fit for the intended application.

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IndEx-2 cell lines

Reporter cell lines

IndEx-2 cell lines

Single-inducible cell line specification sheets:

B7-H3 (CD276)

BCMA

CD19

CHO-K1 IndB7H3: an inducible cell line for the expression of B7H3, for use in the assessment of B7H3-targeted therapeutics.

B7H3 (CD276) is an immune checkpoint molecule that is selectively overexpressed in tumour tissues and immune cells. It is associated with tumour proliferation, metastasis, and therapeutic resistance. This cell line has been developed in a CHO-K1 background and allows inducible expression of B7H3.

CHO-K1 IndB7H3

Inducible Gene: B7-H3 | Species: Human | UniProt: Q5ZPR3


IndEx-2 cell line - B7-H3 histogram and quantification of induction graph RoukenBio.png

Histograms and quantification of B7-H3 induction at 0-100 μM small molecule inducer


Cells were then stained with a labelled antibody and expression levels quantified using a bead-based quantification kit. Specific induction conditions should be further defined by the user. Cell line suitable for research and development use only.

Reporter cell lines

Reporter cell lines are produced and tested in-house:

  • Jurkat NFAT: A Jurkat T-cell line expressing NFAT, designed to monitor T-cell receptor (TCR) activation.
  • Jurkat PD-1/CHO PD-L1 reporter assay: A co-culture of Jurkat PD-1 and CHO PD-L1/TCR activator cells, to assess PD1/PDL1 targeting molecules.
  • Jurkat FcγRIIIa (CD16) V176 NFAT: A Jurkat cell line expressing the FcγRIIIa (CD16) V176 receptor, used to assess CD16 engagement (ADCC).
  • Jurkat FcγRIIIa (CD16) F176 NFAT: A Jurkat cell line expressing the FcγRIIIa (CD16) F176 receptor, used to assess CD16 engagement (ADCC).
  • Jurkat FcγRIIa (CD32a) H167 NFAT: A Jurkat cell line with the FcγRIIa (CD32a) H167 variant, use to assess CD16 engagement (ADCP).
  • Jurkat FcγRI (CD64) NFAT: A Jurkat cell line expressing FcγRI (CD64), designed to monitor Fc receptor signalling (ADCP).
  • HEK293 SRE: A HEK293 cell line engineered to study multiple receptor signalling pathways by monitoring ERK1/2 signalling activation.
  • HEK293 NFκB: A HEK293 cell line used to study various receptor pathways (e.g. TNFR1 or TLRs) via NFκB activation.
  • Jurkat NKG2D reporter assay: A Jurkat cell line expressing the NKG2D allowing the assessment of NKG2D signalling after MICA/B engagement.
  • HEK293 TSLPR/IL-7R STAT5: A HEK293 cell line co-expressing TSLPR and IL-7R, designed to monitor STAT5 signalling in response to receptor activation.
  • HEK293 STAT6: A HEK293 cell line engineered to study IL-4/IL-13 receptor signalling through STAT6 activation.
  • CHO-K1 TrkB NFAT: A CHO cell line expressing the TrkB receptor, used to monitor TrkB signalling pathways via NFAT activation.
  • CHO-K1 Tie Reporter assay: A CHO cell line expressing the Tie2 receptor, designed to monitor Tie2 signalling through an undisclosed pathway.

New cell lines are continuously under development so do not hesitate to contact us for more information.

Jurkat NFAT

Jurkat PD-1/CHO PD-L1 reporter assay

A Jurkat T-cell line expressing NFAT, designed to monitor T-cell receptor (TCR) activation.

Delve into real dual inducible data with IndEx-2

Discover the latest dual inducible IndEx-2 data and dive into two in-depth CAR-T case studies in our exclusive technical slide deck.

Access the slide deck
RoukenBio - IndEx-2 dual inducible cell line system technical presentation.pngcta-image